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hpgk1 luc reporter  (Addgene inc)


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    Structured Review

    Addgene inc hpgk1 luc reporter
    Hpgk1 Luc Reporter, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hpgk1+luc+reporter/pGL4%2E22-PGK1-HRE%3A%3AdLUC+(Plasmid+%23128095)/pm40269168-692-21-29
    Average 93 stars, based on 5 article reviews
    hpgk1 luc reporter - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Activity Assay:

    Article Title: BMAL1–HIF2A heterodimer modulates circadian variations of myocardial injury
    Article Snippet: After 48 h, the culture medium was collected, and the transcriptional activity was quantified using the Secrete-pair Dual Luminescence Assay Kit (GeneCopoeia, LF031) on the Cytation5 (Agilent Technologies) device with Gen5 software (v.3.12, Agilent Technologies). .. To investigate the transcriptional activity of HIF2A and BMAL1 on a general HIF target gene, HEK293 cells were transfected with the hPGK1 -luc reporter (a gift from C. V. Dang, Addgene plasmid, 128095) along with HIF2A or BMAL1 expression constructs. .. After 48 h of transfection, firefly and Renilla luciferase activities were measured using the Dual-Luciferase Reporter Assay System (Promega, E1690).

    Article Title: BMAL1-HIF2A heterodimer modulates circadian variations of myocardial injury.
    Article Snippet: After 48 h, the culture medium was collected, and the transcriptional activity was quantified using the Secrete-pair Dual Luminescence Assay Kit (GeneCopoeia, LF031) on the Cytation5 (Agilent Technologies) device with Gen5 software (v.3.12, Agilent Technologies). .. To investigate the transcriptional activity of HIF2A and BMAL1 on a general HIF target gene, HEK293 cells were transfected with the hPGK1-luc reporter (a gift from C. V. Dang, Addgene plasmid, 128095) along with HIF2A or BMAL1 expression constructs. .. After 48 h of transfection, firefly and Renilla luciferase activities were measured using the Dual-Luciferase Reporter Assay System (Promega, E1690).

    Transfection:

    Article Title: BMAL1–HIF2A heterodimer modulates circadian variations of myocardial injury
    Article Snippet: After 48 h, the culture medium was collected, and the transcriptional activity was quantified using the Secrete-pair Dual Luminescence Assay Kit (GeneCopoeia, LF031) on the Cytation5 (Agilent Technologies) device with Gen5 software (v.3.12, Agilent Technologies). .. To investigate the transcriptional activity of HIF2A and BMAL1 on a general HIF target gene, HEK293 cells were transfected with the hPGK1 -luc reporter (a gift from C. V. Dang, Addgene plasmid, 128095) along with HIF2A or BMAL1 expression constructs. .. After 48 h of transfection, firefly and Renilla luciferase activities were measured using the Dual-Luciferase Reporter Assay System (Promega, E1690).

    Article Title: BMAL1-HIF2A heterodimer modulates circadian variations of myocardial injury.
    Article Snippet: After 48 h, the culture medium was collected, and the transcriptional activity was quantified using the Secrete-pair Dual Luminescence Assay Kit (GeneCopoeia, LF031) on the Cytation5 (Agilent Technologies) device with Gen5 software (v.3.12, Agilent Technologies). .. To investigate the transcriptional activity of HIF2A and BMAL1 on a general HIF target gene, HEK293 cells were transfected with the hPGK1-luc reporter (a gift from C. V. Dang, Addgene plasmid, 128095) along with HIF2A or BMAL1 expression constructs. .. After 48 h of transfection, firefly and Renilla luciferase activities were measured using the Dual-Luciferase Reporter Assay System (Promega, E1690).

    Plasmid Preparation:

    Article Title: BMAL1–HIF2A heterodimer modulates circadian variations of myocardial injury
    Article Snippet: After 48 h, the culture medium was collected, and the transcriptional activity was quantified using the Secrete-pair Dual Luminescence Assay Kit (GeneCopoeia, LF031) on the Cytation5 (Agilent Technologies) device with Gen5 software (v.3.12, Agilent Technologies). .. To investigate the transcriptional activity of HIF2A and BMAL1 on a general HIF target gene, HEK293 cells were transfected with the hPGK1 -luc reporter (a gift from C. V. Dang, Addgene plasmid, 128095) along with HIF2A or BMAL1 expression constructs. .. After 48 h of transfection, firefly and Renilla luciferase activities were measured using the Dual-Luciferase Reporter Assay System (Promega, E1690).

    Article Title: BMAL1-HIF2A heterodimer modulates circadian variations of myocardial injury.
    Article Snippet: After 48 h, the culture medium was collected, and the transcriptional activity was quantified using the Secrete-pair Dual Luminescence Assay Kit (GeneCopoeia, LF031) on the Cytation5 (Agilent Technologies) device with Gen5 software (v.3.12, Agilent Technologies). .. To investigate the transcriptional activity of HIF2A and BMAL1 on a general HIF target gene, HEK293 cells were transfected with the hPGK1-luc reporter (a gift from C. V. Dang, Addgene plasmid, 128095) along with HIF2A or BMAL1 expression constructs. .. After 48 h of transfection, firefly and Renilla luciferase activities were measured using the Dual-Luciferase Reporter Assay System (Promega, E1690).

    Expressing:

    Article Title: BMAL1–HIF2A heterodimer modulates circadian variations of myocardial injury
    Article Snippet: After 48 h, the culture medium was collected, and the transcriptional activity was quantified using the Secrete-pair Dual Luminescence Assay Kit (GeneCopoeia, LF031) on the Cytation5 (Agilent Technologies) device with Gen5 software (v.3.12, Agilent Technologies). .. To investigate the transcriptional activity of HIF2A and BMAL1 on a general HIF target gene, HEK293 cells were transfected with the hPGK1 -luc reporter (a gift from C. V. Dang, Addgene plasmid, 128095) along with HIF2A or BMAL1 expression constructs. .. After 48 h of transfection, firefly and Renilla luciferase activities were measured using the Dual-Luciferase Reporter Assay System (Promega, E1690).

    Article Title: BMAL1-HIF2A heterodimer modulates circadian variations of myocardial injury.
    Article Snippet: After 48 h, the culture medium was collected, and the transcriptional activity was quantified using the Secrete-pair Dual Luminescence Assay Kit (GeneCopoeia, LF031) on the Cytation5 (Agilent Technologies) device with Gen5 software (v.3.12, Agilent Technologies). .. To investigate the transcriptional activity of HIF2A and BMAL1 on a general HIF target gene, HEK293 cells were transfected with the hPGK1-luc reporter (a gift from C. V. Dang, Addgene plasmid, 128095) along with HIF2A or BMAL1 expression constructs. .. After 48 h of transfection, firefly and Renilla luciferase activities were measured using the Dual-Luciferase Reporter Assay System (Promega, E1690).

    Construct:

    Article Title: BMAL1–HIF2A heterodimer modulates circadian variations of myocardial injury
    Article Snippet: After 48 h, the culture medium was collected, and the transcriptional activity was quantified using the Secrete-pair Dual Luminescence Assay Kit (GeneCopoeia, LF031) on the Cytation5 (Agilent Technologies) device with Gen5 software (v.3.12, Agilent Technologies). .. To investigate the transcriptional activity of HIF2A and BMAL1 on a general HIF target gene, HEK293 cells were transfected with the hPGK1 -luc reporter (a gift from C. V. Dang, Addgene plasmid, 128095) along with HIF2A or BMAL1 expression constructs. .. After 48 h of transfection, firefly and Renilla luciferase activities were measured using the Dual-Luciferase Reporter Assay System (Promega, E1690).

    Article Title: BMAL1-HIF2A heterodimer modulates circadian variations of myocardial injury.
    Article Snippet: After 48 h, the culture medium was collected, and the transcriptional activity was quantified using the Secrete-pair Dual Luminescence Assay Kit (GeneCopoeia, LF031) on the Cytation5 (Agilent Technologies) device with Gen5 software (v.3.12, Agilent Technologies). .. To investigate the transcriptional activity of HIF2A and BMAL1 on a general HIF target gene, HEK293 cells were transfected with the hPGK1-luc reporter (a gift from C. V. Dang, Addgene plasmid, 128095) along with HIF2A or BMAL1 expression constructs. .. After 48 h of transfection, firefly and Renilla luciferase activities were measured using the Dual-Luciferase Reporter Assay System (Promega, E1690).



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    93
    Addgene inc hpgk1 luc reporter
    Hpgk1 Luc Reporter, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hpgk1+luc+reporter/pGL4%2E22-PGK1-HRE%3A%3AdLUC+(Plasmid+%23128095)/pm40269168-692-21-29
    Average 93 stars, based on 1 article reviews
    hpgk1 luc reporter - by Bioz Stars, 2026-10
    93/100 stars
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